Selección de bacterias endófitas tipo Bacillus como promotoras de crecimiento en el cultivo de papa variedad Huaycha (Solanum tuberosum subsp. andigena)

Abstract

With the objective of evaluating endophytic bacteria Bacillus type, isolated out native potato plant growth promoters, the Huaycha variety cultivation for the development of bio-products, 39 were activated in-vitro bacterial isolates, of which 30 were found to be Bacillus type. For evaluation purposes, these types were inoculated in greenhouse conditions under completely randomized design with 6 repetitions in two stages blocks. The response variables were: plant height, root size, root weight, root volume, number of tubers, tuber weight and tuber yield.  They were selected 11 isolated as better because they favored greater extent the development of the phenotypic characteristics of the potato crop (13, 19, 27, 32, 38, 45, 46, 47, 138, 146 and 150), the which they were evaluated in-vitro, resulting nitrogen fixers 9,5; 3 phosphorus solubilizing and 3 indol-acetic acid producers. Isolates 27 and 146 met the three functions (fixing of N, P solubilizing and producing AIA) and biochemical characterization showed that correspond to the genus Bacillus. Culminating in the second stage, they were reassessed selecting the best isolates (19, 146, 45, 47 and 150 including the commercial inoculum Bs) in greenhouse, where he presented greater effect on plant development. Stading out isolated 45, to present greater plant height and tuber development with a yield of 3 t/ha of potatoes.